Tag Archives: herbicide resistance

Identification of Herbicide Resistant Weeds in NZ

Chris Buddenhagen told the folk at the 2022 LandWISE AGM Seminar in late June about research progress in the AgResearch Managing Herbicide Resistance programme,

Working with FAR, the Bragato Research Centre, and Massey University, AgResearch scientists are surveying arable farms and vineyards for presence of herbicide resistant weeds. They have collected seed from across Aotearoa including Canterbury, Otago, Marlborough, Waikato, Bay of Plenty, Gisborne, Hawke’s Bay and Wairarapa.

The first time a deliberate survey looking for examples of herbicide resistance has been conducted, the research is adding to the number of known cases in New Zealand.  As can be seen in the chart below, grasses feature prominently, some to several herbicide groups.

Chris explained that resistance results from plants with certain genes or sets of genes being preferentially selected by the management to which they are exposed. Plants constantly mutate, and a constant exposure to a certain stressor (in this case a herbicide group) means those that cope best will survive and, over time, become the dominant population. 

To identify resistance, seed has been collected from suspected survivors and from soil samples taken from the survey sites.  The seeds are germinated in a greenhouse, and sown in trays for testing.

Once established, the trays are sprayed at a defined spray rate, and plants observed for about three to six weeks. 

After a few weeks, the results of spraying become apparent. Some lines are shown to be susceptible to a particular spray, and others resistant.

Identifying resistance this way is slow and expensive. The project is also investigating other rapid testing techniques including the use of genetic markers so any plant sample can be tested virtually overnight, and at much lower cost. But that’s a story for another day.

If you want to read about the Herbicide Survey work in detail, information from the first surveys is available online as an open access publication.

See:  Buddenhagen CE, James TK, Ngow Z, Hackell DL, Rolston MP, Chynoweth RJ, Gunnarsson M, Li F, Harrington KC, Ghanizadeh H. 2021.  Resistance to post-emergent herbicides is becoming common for grass weeds on New Zealand wheat and barley farms.  Uludag A, editor. PLoS ONE. 16(10):e0258685. https://doi.org/10.1371/journal.pone.0258685

We thank Chris, Trevor James and all involved in the work for presenting it to the LandWISE Seminar participants.

Herbicide Resistance

Herbicide resistant weeds are a real and increasing issue globally and evident in New Zealand. Herbicide resistant ryegrass is for example, a problem in both arable farms and vineyards.

Atrazine resistant Amaranthus (Trevor James photo)

We are working with Trevor James and AgResearch in a project focused on improved weed control and vegetation management to minimise future herbicide resistance. The project is funded through the Ministry of Business, Innovation and Employment (MBIE) and major co-funder, the Foundation for Arable Research (FAR).

The project has four main work areas:

A Lincoln University team is seeking to identify the weeds most likely to develop herbicide resistance in new regions. Outputs will be a risk index that indicates weeds with a history of herbicide resistance, herbicide resistant weeds that pose the greatest risk if introduced and weeds that have a high likelihood of becoming resistant.

An AgResearch team seeks to identify and describe the drivers of on- and off-farm herbicide practices to more successfully address factors across the supply/value chain that increase the risk of herbicide resistance.

Grasslands and Massey University researchers will develop genotyping and seed bioassays to create ‘quick tests’ for resistance in key weed species. They will also model spread scenarios for resistance genes to determine the greatest risk of resistance i.e. from resistance developing on-site or from dispersal of resistant weeds. They are starting with perennial ryegrass before adding other species for screening.

We are in a team led by Trevor James looking to develop new non-herbicidal interventions (e.g. robotic weeders, abrasion technologies and smart cultivators) and the use of cover crops (in collaboration with FAR) for both managing existing and avoiding new instances of herbicide resistance.

Included in this section is ‘rediscovering’ Māori management practices such as traditional strategic resting and natural pathogenic organisms to target the soil weed seed bank. While virtually all our problem weeds are introduced from Europe and the Americas, the holistic approaches typical in Māoridom seem fully relevant to a systems based approach to weed management. A second group in this team is to isolate and evaluate natural pathogenic fungi and bacteria for their ability and efficacy to kill weed seeds.

LandWISE members are well-aware of the risks of herbicide resistance. It has been an aspect of LandWISE projects since the early 2000s when we began promoting strip tillage and no-till systems to maintain soil quality and reduce energy inputs. The extra pressure on herbicide controls when physical cultivation is reduced saw us publish charts of herbicide groups for different crops. Maybe it is time that work was brought up to date!

More information about this project is available on our project pages here>